Scientific Program
Advanced Column Technologies
This session will focus on presenting recent progress on new column technologies or use of such in bioanalysis. Although not restricted to it, the emphasis is placed on micro and nanoscale separation formats. We aim to have interactive discussions with symposium presenters while outlining future trends in column development.
Biologics/Glycan Analysis
This session will focus on advances in microscale and hyphenated separations (both chromatographic and electrophoretic) for the characterization and analysis of biopharmaceuticals. Fundamental aspects, new methodologies and high-impact applications will be covered.
CE-MS/Novel Detection Strategies
This session focuses on the interface of capillary electrophoresis with mass spectrometry and other detection technologies. The program considers both new interface technologies and their application to analytical problems.
Clinical Diagnostics & POC Devices
This session will focus on novel technologies and devices that enable near patient clinical diagnostic applications. The session will feature micro-scale separation and bioanalysis approaches to detect and characterize biomarkers such as small molecules, proteins, nucleic acids, and cells, from relevant sample matrices such as blood or urine, in a format that is suitable for point of care settings.
Comprehensive “-OMICS”
This session will focus on recent innovations in high efficiency separation techniques, covering state-of-the-art (miniaturized) chromatographic and electrophoretic methods coupled to mass spectrometry for untargeted analysis of proteins, lipids and metabolites. Complementary separation modes and multidimensional separations that expand peak capacity will also be considered, including strategies for unknown identification.
Environmental Analysis
The session will highlight state-of-the-art techniques and methodologies for the separation, identification and quantification of environmental contaminants. Topics will include traditional targeted and non-targeted analytical techniques as well as new strategies to assess environmental impact, exposure, and health effects.
Food, Nutrition & Health
This session will focus on nutritional studies demonstrating the health-promoting and/or disease-preventing properties of functional foods using animal and human models. The application of micro-scale separation and bioanalysis techniques to characterize bioactive compounds and their effects on endo- and exogenous metabolites in biological samples will be discussed.
Fundamentals & Biointeractions
Advances in micro-scale separations benefit from understanding chemical equilibrium and kinetics, molecular transport, fluid dynamics, heat transfer, and signal-to-noise theory. This session will include presentations that apply fundamentals to the solution of separation and instrumentation problems.
Future Stars in Microseparations
The session will showcase exciting research contributions from young investigators/early career researchers from across the globe with a focus on the basic development and/or innovative applications of microscale separations for bioanalysis. To qualify, abstract submissions must be from post-doctoral researchers or tenure-track assistant professors within five years of their first appointment.
Ion Mobility Spectrometry
Rapid advances in ion mobility-mass spectrometry (IM-MS) have uniquely positioned the technique for small volume and high throughput analysis of complex biological systems. Ultra-fast electrophoretic gas-phase separations on the basis of molecular structure are afforded by IM, while accurate mass data is provided by the MS, respectively. This session will focus on emerging instrumental configurations and biological applications of IM-MS separation methods.
From the ‘Stanford GC’ device in 1978 to the evolution of ‘micro-TAS’ and CE-based systems in the early 1990s, separations science has led the way in the microfluidic revolution. This session will feature the latest in separations enabled by microfluidics, including innovations in packing materials, separation mechanisms, device designs, and detectors.
Multidimensional Separations
This session will be devoted to all aspects of comprehensive multidimensional separations, covering techniques like GC×GC, LC×LC, etc. Both fundamental aspects and applications of the techniques will be presented by experts in the field.
Nucleic Acids, Biosensors & Forensics
This session explores various aspects of nucleic acids in biosensing, bioseparation, and forensic applications. Research topics in this session include, but are not limited analytical and sequencing techniques for genomics/epigenetics, biosensors on the basis of hybridization assays, nanomaterials, and nanofabrication, microfluidics and rapid nucleic acid analysis, aptamers and biosensors for non-nucleic acid targets, as well as forensic genetics, barcode of life, and other forensic-related techniques.
Pharmaceutical & Chiral Analysis
This session aims at bringing analytical scientists from academia and industry together to discuss the newest developments in pharmaceutical analysis and chiral separations. Oral presentations on relevant topics, such as new strategies in method development and method validation, novel separation media for pharmaceutical and chiral separations, new trends in sample preparation of pharmaceuticals, improved detection strategies for pharmaceutical analysis and methodological aspects will be discussed.
Sample Preparation
This session will focus on all aspects of microscale sample preparation in food, environmental and bioanalysis with topics ranging from microextraction, dried blood spot sampling and micro solid-phase extraction approaches to new materials, methods and devices, including microreactors. Sample preparation plays a critical role in -omics applications, so novel state-of-the art approaches to improve sample preparation for metabolomics and proteomics will also be discussed.
Poster Finalists: 3 Minute Talks
This special session will be devoted to our top ranked poster finalists who will concisely present their research in a 3 minute talk (3MT) to delegates at the conference.
Short Courses
Short Courses are run in combination as two parallel sessions. Courses run for 3.5 hours (1.5 each with 30 min coffee break in between). Courses are offered only on Sunday, April 3 from 1:00 pm to 4:30 pm.
Option A

A1 - Sampling and Sample Preparation for Bioanalysis,
presented by: Janusz Pawliszyn, University of Waterloo

During the course solventless sampling/sample preparation method, solid phase microextraction (SPME) will be discussed. This course will cover the main principles of the technique, calibration methods, coupling strategies of SPME to GC, LC, CE and MS, method development strategy, as well as advantages and disadvantages of the technique.  Applications of the technologies in the fields such as forensic, pharmaceutical and clinical analysis will be discussed. Advances in the methods will be highlighted including: ligand-receptor binding and plasma protein binding studies, breath and skin analysis, in vivo sampling of freely moving animals for pharmacokinetic and metabolomic studies, automation in high-throughput format, direct tissue analysis with particular focus on intraoperative monitoring of drugs and biomarkers.

A2 -
Bioinformatics and Statistical Methods for Metabolomics,
presented by: David Wishart, University of Alberta

The workshop will cover many topics ranging from understanding metabolomics technologies, data collection and analysis, using pathway databases, performing pathway analysis, conducting univariate and multivariate statistics, working with metabolomic databases and exploring chemical databases. Participants will be given various data sets and short assignments to assist with the learning process.

Option B

B1 - Quality by Design for Analytical Method Development,
presented by: Cari Sanger, Kantisto BV

QbD is a scientific, risk-based, holistic and proactive approach originally defined for product development. QbD works with predefined objectives and emphasizes product and process understanding and process control. In short, QbD is based on sound science and quality risk management. This is just as valid for analytical method development and AQbB has been adapted for several years in the (bio)pharma industry. The course will cover the main aspects of AQbD and explain the QbD language. Several examples will be shown and we will discuss how to make sure that we are developing reliable, efficient, and robust cost-saving methods to ensure that quality and patient safety standards are met.

B2 - New Advances in Biologics/Biopharmaceutical Characterization using CE and CE-MS,
presented by: G.W. Somsen, R. Haselberg, Vrije Universiteit - Amsterdam

CE has gained widespread interest in the analysis of protein-based therapeutics. Today, CE-SDS, CIEF and CE-LIF are routinely used for assessing the size, charge and glycan heterogeneity of protein pharmaceuticals. This course will start by treating the main aspects of these established CE techniques, and then focus on key areas where CE and CE‒MS can further enhance the characterization of biologics and biosimilars. Attention will be paid to capillary coating, MS interfacing and practical conditions used for intact protein analysis. The capability of CZE and CZE‒MS to probe modifications, such as deamidation, acetylation, lysine clipping, glycation and pyroglutamate formation, will be discussed. The potential of CE(‒MS) for biopharmaceutical characterization will be outlined by examples covering glycosylated biopharmaceuticals, monoclonal antibodies, antibody-drug conjugates (ADCs) and vaccine formulations.